A Method for High Efficiency YAC Lipofection into Murine Embryonic Stem Cells
نویسندگان
چکیده
منابع مشابه
A method for high efficiency YAC lipofection into murine embryonic stem cells.
We describe a modified protocol for introducing yeast artificial chromosomes (YACs) into murine embryonic stem (ES) cells by lipofection. With a decreased DNA:cell ratio, increased concentration of condensing agents and altered culture conditions, this protocol reduces the requirement for YAC DNA to a few micrograms, improves the recovery of neomycin-resistant ES colonies and increases the yiel...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/24.24.5054